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abcg2  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec abcg2
    Abcg2, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd338/CD338+(ABCG2)+Antibody%2C+anti-human/pm41827828-94-4-27
    Average 94 stars, based on 27 article reviews
    abcg2 - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    Expressing:

    Article Title: Dual gene activation and knockout screen reveals directional dependencies in genetic networks
    Article Snippet: .. Four weeks post introduction of the SaCas9 expression cassette, clonal lines were screened for functionality of both CRISPR systems (CRISPRa and SaCas9 nuclease) via flow cytometry analysis of >10,000 cells stained with CD338 ( ABCG2 ) antibodies (Miltenyi, 130-104-960). ..

    CRISPR:

    Article Title: Dual gene activation and knockout screen reveals directional dependencies in genetic networks
    Article Snippet: .. Four weeks post introduction of the SaCas9 expression cassette, clonal lines were screened for functionality of both CRISPR systems (CRISPRa and SaCas9 nuclease) via flow cytometry analysis of >10,000 cells stained with CD338 ( ABCG2 ) antibodies (Miltenyi, 130-104-960). ..

    Flow Cytometry:

    Article Title: Dual gene activation and knockout screen reveals directional dependencies in genetic networks
    Article Snippet: .. Four weeks post introduction of the SaCas9 expression cassette, clonal lines were screened for functionality of both CRISPR systems (CRISPRa and SaCas9 nuclease) via flow cytometry analysis of >10,000 cells stained with CD338 ( ABCG2 ) antibodies (Miltenyi, 130-104-960). ..

    Article Title: Preterm Cord Blood Contains a Higher Proportion of Immature Hematopoietic Progenitors Compared to Term Samples
    Article Snippet: To set the gates for SP cells, an aliquot of cells was pre-incubated with 50 μM Verapamil (Sigma-Aldrich) for 30 min at 37°C before staining with DCV. .. At the end of the incubation, cells were labeled with anti-CD34 (BD Biosciences), CD338 (ABCG-2; Miltenyi Biotechnology) and 7-AAD (EBioScience) and subsequently detected on an LSRFortessa flow cytometer (BD Biosciences). .. Results wereanalyzed using FlowJo software according to the gating strategy previously described [ ].

    Staining:

    Article Title: Dual gene activation and knockout screen reveals directional dependencies in genetic networks
    Article Snippet: .. Four weeks post introduction of the SaCas9 expression cassette, clonal lines were screened for functionality of both CRISPR systems (CRISPRa and SaCas9 nuclease) via flow cytometry analysis of >10,000 cells stained with CD338 ( ABCG2 ) antibodies (Miltenyi, 130-104-960). ..

    Article Title: Repression of PPARγ reduces the ABCG2-mediated efflux activity of M2 macrophages.
    Article Snippet: Even though subclasses of macrophage have distinct roles during progression of infectious diseases, it remains poorly understood whether there is a subset-specific difference in drug responses.. Here, we report that ABCG2 was expressed specifically in M2-like macrophages and that it controlled their efflux activities.. Abcg2 expression is markedly induced during polarization of PMA-primed macrophages toward an M2 type.

    Incubation:

    Article Title: Repression of PPARγ reduces the ABCG2-mediated efflux activity of M2 macrophages.
    Article Snippet: Even though subclasses of macrophage have distinct roles during progression of infectious diseases, it remains poorly understood whether there is a subset-specific difference in drug responses.. Here, we report that ABCG2 was expressed specifically in M2-like macrophages and that it controlled their efflux activities.. Abcg2 expression is markedly induced during polarization of PMA-primed macrophages toward an M2 type.

    Article Title: Preterm Cord Blood Contains a Higher Proportion of Immature Hematopoietic Progenitors Compared to Term Samples
    Article Snippet: To set the gates for SP cells, an aliquot of cells was pre-incubated with 50 μM Verapamil (Sigma-Aldrich) for 30 min at 37°C before staining with DCV. .. At the end of the incubation, cells were labeled with anti-CD34 (BD Biosciences), CD338 (ABCG-2; Miltenyi Biotechnology) and 7-AAD (EBioScience) and subsequently detected on an LSRFortessa flow cytometer (BD Biosciences). .. Results wereanalyzed using FlowJo software according to the gating strategy previously described [ ].

    Control:

    Article Title: Repression of PPARγ reduces the ABCG2-mediated efflux activity of M2 macrophages.
    Article Snippet: Even though subclasses of macrophage have distinct roles during progression of infectious diseases, it remains poorly understood whether there is a subset-specific difference in drug responses.. Here, we report that ABCG2 was expressed specifically in M2-like macrophages and that it controlled their efflux activities.. Abcg2 expression is markedly induced during polarization of PMA-primed macrophages toward an M2 type.

    Labeling:

    Article Title: Preterm Cord Blood Contains a Higher Proportion of Immature Hematopoietic Progenitors Compared to Term Samples
    Article Snippet: To set the gates for SP cells, an aliquot of cells was pre-incubated with 50 μM Verapamil (Sigma-Aldrich) for 30 min at 37°C before staining with DCV. .. At the end of the incubation, cells were labeled with anti-CD34 (BD Biosciences), CD338 (ABCG-2; Miltenyi Biotechnology) and 7-AAD (EBioScience) and subsequently detected on an LSRFortessa flow cytometer (BD Biosciences). .. Results wereanalyzed using FlowJo software according to the gating strategy previously described [ ].



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    E T9I has increased affinity to autophagosome-associated proteins (A) Principal component analysis of the differential interactome data , the individual replicates are separated (black: GFP controls, Green: E T9 pulldown, Purple: E T9I pulldown) (B) Volcano plot of the differential interactome analysis showing enriched proteins in E T9I pulldown versus the p value (-log P). Five highly significantly enriched proteins are highlighted in red and via labels. (C–G) Quantification of proximity ligation assays between transiently expressed SARS-CoV-2 E variants 30 h post transfection in HeLa cells and endogenous SNX12, STX12, TMEM87B, <t>ABCG2</t> and TAB1, as indicated. Representative images depicted. PLA signal, red. Scale Bar, 10μm. DAPI, nuclei (blue). Lines represent the mean of N = 18–59 (individual cells) ±SEM. (H) Quantification of autophagosome levels by flow cytometry in HEK293T autophagy reporter cells (HEK293T-GL) transiently expressing StrepII-tagged SARS-CoV-2 E variants (48 h post transfection) and depleted of indicated proteins by siRNA. Bars represent the mean of N = 3 (biological replicates) ±SEM. Student’s t test with Welch’s correction. ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001.
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    Novus Biologicals specificity to bcrp
    Figure 3. TFV/FTC efflux transporters and nucleotide-metabolizing kinases are differentially active in BBB cells. Brain microvascular endothelial cell, pericyte, and astrocyte monocultures were loaded with dyes for (A) P-gp (rhodamine 123, 10 μM), <t>(B)</t> <t>MRP4</t> (monobromobimane, 10 μM), and (C) <t>BCRP</t> (Hoechst 33342, 5 μg/mL) for 15 min at 37 °C, 5% CO2. The dyes were allowed to efflux out for 2 h at 37 °C and 5% CO2, after which flow cytometry was performed to quantify intracellular fluorescence (mean fluorescence intensity, MFI) as an indicator of efflux capacity. Statistical analysis was performed using one-way ANOVA. (D) P-gp and (E) BCRP expressions were confirmed by Western blot, as these proteins were not reliably measurable by proteomics analyses. (F) Endogenous PK and (H) TK activities were measured in primary human BBB cell monocultures by a colorimetric activity assay and DiviTum activity assay, respectively. Four to eight independent experiments (represented by individual dots) were performed. Statistical analysis was performed using a Brown−Forsythe and Welch ANOVA test. (G) TFV-DP formation in human primary BBB cell lysates was measured by a CKB-mediated TFV metabolism assay using LC−MS/MS. Cell lysates were incubated with TFV-monophosphate (TFV-MP) and phosphocreatine (+CKB Substrates) or a mixture of phosphocreatine, phosphoenolpyruvate, and ATP (+Phosphate Donors) for 30 min at 37 °C. The reaction was quenched by LC−MS-grade methanol, and peak area of TFV-DP was measured by LC−MS/MS. Three to five independent experiments were performed (represented by individual dots). Statistical analysis was performed by using one-way ANOVA by GraphPad software. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.
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    Image Search Results


    E T9I has increased affinity to autophagosome-associated proteins (A) Principal component analysis of the differential interactome data , the individual replicates are separated (black: GFP controls, Green: E T9 pulldown, Purple: E T9I pulldown) (B) Volcano plot of the differential interactome analysis showing enriched proteins in E T9I pulldown versus the p value (-log P). Five highly significantly enriched proteins are highlighted in red and via labels. (C–G) Quantification of proximity ligation assays between transiently expressed SARS-CoV-2 E variants 30 h post transfection in HeLa cells and endogenous SNX12, STX12, TMEM87B, ABCG2 and TAB1, as indicated. Representative images depicted. PLA signal, red. Scale Bar, 10μm. DAPI, nuclei (blue). Lines represent the mean of N = 18–59 (individual cells) ±SEM. (H) Quantification of autophagosome levels by flow cytometry in HEK293T autophagy reporter cells (HEK293T-GL) transiently expressing StrepII-tagged SARS-CoV-2 E variants (48 h post transfection) and depleted of indicated proteins by siRNA. Bars represent the mean of N = 3 (biological replicates) ±SEM. Student’s t test with Welch’s correction. ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001.

    Journal: iScience

    Article Title: Mutation T9I in Envelope confers autophagy resistance to SARS-CoV-2 Omicron

    doi: 10.1016/j.isci.2025.112974

    Figure Lengend Snippet: E T9I has increased affinity to autophagosome-associated proteins (A) Principal component analysis of the differential interactome data , the individual replicates are separated (black: GFP controls, Green: E T9 pulldown, Purple: E T9I pulldown) (B) Volcano plot of the differential interactome analysis showing enriched proteins in E T9I pulldown versus the p value (-log P). Five highly significantly enriched proteins are highlighted in red and via labels. (C–G) Quantification of proximity ligation assays between transiently expressed SARS-CoV-2 E variants 30 h post transfection in HeLa cells and endogenous SNX12, STX12, TMEM87B, ABCG2 and TAB1, as indicated. Representative images depicted. PLA signal, red. Scale Bar, 10μm. DAPI, nuclei (blue). Lines represent the mean of N = 18–59 (individual cells) ±SEM. (H) Quantification of autophagosome levels by flow cytometry in HEK293T autophagy reporter cells (HEK293T-GL) transiently expressing StrepII-tagged SARS-CoV-2 E variants (48 h post transfection) and depleted of indicated proteins by siRNA. Bars represent the mean of N = 3 (biological replicates) ±SEM. Student’s t test with Welch’s correction. ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001.

    Article Snippet: Monoclonal rabbit anti-ABCG2 Antibody (2K8X1) (WB 1:500) , Novus Biologicals , Cat#NBP3-15559.

    Techniques: Ligation, Transfection, Flow Cytometry, Expressing

    Figure 3. TFV/FTC efflux transporters and nucleotide-metabolizing kinases are differentially active in BBB cells. Brain microvascular endothelial cell, pericyte, and astrocyte monocultures were loaded with dyes for (A) P-gp (rhodamine 123, 10 μM), (B) MRP4 (monobromobimane, 10 μM), and (C) BCRP (Hoechst 33342, 5 μg/mL) for 15 min at 37 °C, 5% CO2. The dyes were allowed to efflux out for 2 h at 37 °C and 5% CO2, after which flow cytometry was performed to quantify intracellular fluorescence (mean fluorescence intensity, MFI) as an indicator of efflux capacity. Statistical analysis was performed using one-way ANOVA. (D) P-gp and (E) BCRP expressions were confirmed by Western blot, as these proteins were not reliably measurable by proteomics analyses. (F) Endogenous PK and (H) TK activities were measured in primary human BBB cell monocultures by a colorimetric activity assay and DiviTum activity assay, respectively. Four to eight independent experiments (represented by individual dots) were performed. Statistical analysis was performed using a Brown−Forsythe and Welch ANOVA test. (G) TFV-DP formation in human primary BBB cell lysates was measured by a CKB-mediated TFV metabolism assay using LC−MS/MS. Cell lysates were incubated with TFV-monophosphate (TFV-MP) and phosphocreatine (+CKB Substrates) or a mixture of phosphocreatine, phosphoenolpyruvate, and ATP (+Phosphate Donors) for 30 min at 37 °C. The reaction was quenched by LC−MS-grade methanol, and peak area of TFV-DP was measured by LC−MS/MS. Three to five independent experiments were performed (represented by individual dots). Statistical analysis was performed by using one-way ANOVA by GraphPad software. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.

    Journal: ACS pharmacology & translational science

    Article Title: All Blood Brain Barrier Cell Types Demonstrate Capability to Influence Differential Tenofovir and Emtricitabine Metabolism and Transport in the Brain.

    doi: 10.1021/acsptsci.4c00510

    Figure Lengend Snippet: Figure 3. TFV/FTC efflux transporters and nucleotide-metabolizing kinases are differentially active in BBB cells. Brain microvascular endothelial cell, pericyte, and astrocyte monocultures were loaded with dyes for (A) P-gp (rhodamine 123, 10 μM), (B) MRP4 (monobromobimane, 10 μM), and (C) BCRP (Hoechst 33342, 5 μg/mL) for 15 min at 37 °C, 5% CO2. The dyes were allowed to efflux out for 2 h at 37 °C and 5% CO2, after which flow cytometry was performed to quantify intracellular fluorescence (mean fluorescence intensity, MFI) as an indicator of efflux capacity. Statistical analysis was performed using one-way ANOVA. (D) P-gp and (E) BCRP expressions were confirmed by Western blot, as these proteins were not reliably measurable by proteomics analyses. (F) Endogenous PK and (H) TK activities were measured in primary human BBB cell monocultures by a colorimetric activity assay and DiviTum activity assay, respectively. Four to eight independent experiments (represented by individual dots) were performed. Statistical analysis was performed using a Brown−Forsythe and Welch ANOVA test. (G) TFV-DP formation in human primary BBB cell lysates was measured by a CKB-mediated TFV metabolism assay using LC−MS/MS. Cell lysates were incubated with TFV-monophosphate (TFV-MP) and phosphocreatine (+CKB Substrates) or a mixture of phosphocreatine, phosphoenolpyruvate, and ATP (+Phosphate Donors) for 30 min at 37 °C. The reaction was quenched by LC−MS-grade methanol, and peak area of TFV-DP was measured by LC−MS/MS. Three to five independent experiments were performed (represented by individual dots). Statistical analysis was performed by using one-way ANOVA by GraphPad software. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.

    Article Snippet: Western blots were performed as previously described.16 Blots were probed with antibodies with specificity to BCRP (NBP2-22124, Novus Bio, Centennial, CO), P-gp (PA5-61300, Invitrogen), MRP4 (12705, Cell Signaling Technology), MRP1 (Ab24102, Abcam), ENT1 (PA5-116451, Invitrogen), PGK1 (PA528612, Invitrogen), AK2 (11014-1-AP, Proteintech, Rosemont, IL), CKB/CKM (15137-1-AP, Proteintech), TK (15691-1-AP, Proteintech), CMPK (11360-1-AP, Proteintech), DCK (17758-1-AP, Proteintech), PKM1 (15821-1-AP, Proteintech), and PKLR (2456-1-AP, Proteintech), overnight at 4 °C, washed with TBS-T, and probed with the appropriate secondary antibody (ab97023 and Ab97051, Abcam) for 1 h at room temperature.

    Techniques: Flow Cytometry, Fluorescence, Western Blot, Activity Assay, Liquid Chromatography with Mass Spectroscopy, Incubation, Software